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If you’ve got a question, then there’s a good chance we’ve got the answer here for you.

1What are probiotics?

Probiotics are microbes that are literally everywhere including the inside and outside of our bodies. They are good bacteria that reduce the germ population naturally, and safely, creating a more healthful environment. The definition of probiotic by the World Health Organization is “For life.”

2What is PIP?

Chrisal’s PIP (Probiotics in Progress) product line represents a new generation of probiotic hygiene products containing good bacteria that displace pathogenic (bad) bacteria.

3How does Probiotic cleaning work?

Unlike disinfectants, antimicrobial soaps, biocides or other chemicals that destroy all of the microorganisms (good and bad) present, Chrisal’s probiotic cleaners create a healthy microbial balance, without killing anything. Following the natural principle of competitive exclusion, millions of tiny probiotic workers flood the surface, consume all of the resources, and crowd out unwanted germs and contaminants. These beneficial bacteria also provide a safe layer of protection, lasting for up to 3 days.

4Where can PIP products be used?

Chrisal’s probiotic products are applicable anyplace where hygiene is important!

  • Hospitals and healthcare facilities – nursing homes, medical clinics, etc.
  • Residential and commercial buildings
  • Schools and institutions
  • Sports and recreational facilities
  • Offices and workplaces
  • Farms and animal housing – horses, cows, pigs, chickens, dairy
  • Water systems
  • Private homes

5Do I keep disinfecting when using PIP Products?

No, the best results with PIP products are obtained without disinfection. However, when certain conditions mandate disinfection, such as a MRSA contaminated room, we recommend a PIP treatment immediately following disinfection to protect the area from further contamination.

6Are PIP products safe?

Due to their biological nature, all PIP products are perfectly safe, environmentally friendly, beneficial and harmless. While maintaining exceptional cleaning power, Chrisal’s probiotic product line is so gentle on skin that it can be used without gloves, masks or protective aprons. For additional information on the safety aspects of our products, please contact us for our product MSDS’s.

7What is competitive exclusion?

The competitive exclusion principle, also called Gause’s Law, states that when two species compete for the same critical resources, typically food and moisture, one will eventually outcompete and displace the other. The displaced species will become locally extinct, by either migration or death.

8How do Chrisal PIP products competitively exclude bacteria?

hrisal PIP products guarantee a minimum of 50 million cfu/ml (colony-forming units per ml). When applied to a surface, tens of millions of tiny probiotic workers flood the area, gobble up dirt and contaminants, deconstruct biofilm and claim dominance of the space.

9What is quorum sensing?

Quorum sensing is a form of communication between bacteria. Instead of language, bacteria use signaling molecules to coordinate their behaviour. As environmental conditions often change rapidly, bacteria need to respond quickly in order to survive. These responses include adaptation to the availability of nutrients, defense against other microorganisms competing for the same nutrients, and the avoidance of toxic compounds potentially dangerous for the bacteria. As well as releasing the signaling molecules, bacteria are also able to measure the number (concentration) of the molecules within a population.

10How does quorum sensing relate to PIP products?

When Chrisal probiotic bacteria are applied to a surface, there is an overwhelming competition for space, food and moisture. There is also a flood of signalling molecules between bacteria, informing each other that conditions are unfavourable for growth. As a result, they enter a dormant phase, leave or die.

11What is a microbe?

A microbe is a microorganism. Other synonyms include germ, virus and bacteria. Microbes are the building blocks of biofilms.

12What is a pathogen?

The first link in the chain of infection, a pathogen is anything that causes disease in humans, animals, birds and fish. Here are some examples of pathogens:

  • Bacteria: Microbes that are capable of reproducing on their own, causing human disease by direct invasion of body tissues. Bacteria often produce toxins that poison the cells they have invaded. Numerous bacteria also live in harmony with the body and are necessary for human existence, such as bacteria that aid in digestion in the gut. Important bacterial diseases include “strep” tonsillitis, pneumonia, and meningitis.
  • Virus: Microbes that are incapable of reproducing on their own, and must invade a host cell in order to use its genetic machinery for reproduction. Viruses are smaller than bacteria, and are responsible for the most common human diseases, including colds and the “flu” (influenza). Viruses are also responsible for more serious diseases such as AIDS, hepatitis B, and hepatitis C.
  • Fungus: A large group of organisms that includes microbes such as yeast and mold. Other examples of pathogenic fungus are Athlete’s Foot and Candida.

13What is biofilm?

Biofilm is an aggregation of microbes, a tiny community of living microorganisms that grow on living or inert surfaces and surround themselves in a thick, adhesive substance or matrix.

Ubiquitous in the environment, biofilms form on our teeth, inside our bodies, in our streams and oceans, on natural surfaces continually wetted by dripping water, and on surfaces in our homes and workplaces. Biofilms also are formed inside of all of our water pipes, toilets, and drains, and, anywhere there is persistent water.

14How are biofilms formed?

Biofilms are formed when bacteria adhere to a solid surface and enclose themselves in a sticky polysaccharide. Once this polysaccharide is formed the bacteria can no longer leave the surface, and when new bacteria are produced they stay within this sticky layer.

15What is the function of biofilms?

Biofilms trap dirt, viruses, pathogens and other contaminants. This slimy matrix is the key weapon microorganisms use to defeat the immune system, disinfectants, antibiotic drugs, and other threats. Within the protective barrier of biofilms, pathogens aggregate and multiply, greatly increasing virulence.

16Why are biofilms a problem?

The primary source of viral or bacterial disease transmission is surface-to-hand or hand-to-hand contact. A virus is incapable of living away from a host
 so if it lands on a surface without a support system, it may die within hours. A bacterium has a protective coating that gives it a longer life span but, without a support system, it too will die. However, if either of these pathogens land on a biofilm, they can remain alive and virulent for a long, long time.

In many cases, the key to infection protection and reduction is to attack and eradicate biofilm. It is now generally accepted that the biofilm growth mode induces microbial resistance to disinfection that can lead to substantial economic and health concerns.

To appreciate the concerns presented by biofilms, check out this article: Bacterial Biofilms – why should we care?

http://www.cresa.cat/blogs/sociedad/en/espanol-biofilms-bacterianos-por-que-deberia-importarnos/

17Where are biofilms in your home?

Biofilms colonize many household surfaces in the bath and kitchen, including toilets, sinks, countertops, and cutting boards. They are also present in areas where there is persistent water. Here are some visual examples: http://www.biofilm.montana.edu/content/household-biofilms

18What health problems can biofilms cause?

Biofilms are implicated in more than 80% of chronic inflammatory and infectious diseases caused by bacteria. They are known to cause a variety of health problems, ranging from a common earache to a specific bacterial infection found in people living with a genetic disease called cystic fibrosis.

19Is it easy to eliminate of biofilms?

Getting rid of biofilms is not an easy task; they won’t go away on their own, and considerable effort is required to eliminate them. Biofilms on your teeth, which contribute to plaque formation and tooth decay, are removed by diligent scrubbing with somewhat abrasive materials. Unfortunately, the biofilms return within hours, making teeth cleaning an endless process.

Using conventional cleaning methods in an effort to remove biofilms from surfaces is also a never-ending endeavour. While detergents and enzymes cut through some of the layers in these slimy strongholds, destroying trapped bacteria takes more firepower, such as an army of probiotic workers.

20What is the problem with disinfectants?

Disinfectants and biocides may provide a deep cleaning effect, but they do not remove all of the dirt and contaminants down to the microscopic level. They also stop working the minute they are dry! Even the strongest disinfectants that may kill most of the bacteria, don’t physically remove all of the ‘dead bodies’ left after the chemical attack. Instead, this organic debris becomes food and facilitates rapid growth of the next round of bacterial invaders.

21What causes resistance to disinfectants?

The resistance of microorganisms to disinfection is frequently associated with the presence of biofilms on surfaces. The multiple layers of cells in this sticky, complex structure are difficult for disinfectants and biocides to penetrate. As a result, biofilms are highly resistant to traditional cleaning and disinfection protocols.

22Don’t harmful germs become resistant to probiotics?

No. Microbes cannot become resistant to other microbes, only to the chemicals that threaten them. There are no chemicals in PIP products so no resistance can be developed.

23How do PIP products help ponds and aquariums?

PIP deconstructs biofilm, unclogging filters and clarifying the water. The healthy microbial balance improves the food conversion rate for fish.

24How serious is the problem of infection control?

It’s a problem that appears to be getting worse. Consider these points:

  • Infection control is a hot topic with hospital-acquired infections being challenged by antibiotic resistant Superbugs.
  • The Center for Disease Control recently voiced concerns about future availability of effective antibiotics.
  • The World Health Organization’s 2014 report states that drug-resistant Superbugs are now a global threat.
  • New treatments to fight Superbugs are estimated to be 10 years away.
  • Gyms and fitness centres are a source of pathogens, including MSRA.
  • Parents need to investigate infection control policies in daycare and schools to ensure that their children are safe.
  • The FDA says that soap and water is as effective as antimicrobial soaps that create resistance.
  • Studies have found that between 50 and 80 percent of chickens sold at US retail locations are contaminated with Campylobacter, while approximately 15 percent carry Salmonella. This study was the first to examine the relationship between pathogen loads (the amount of the pathogens found) at the farm and at the processing plant.

25Are Chrisal bacteria aerobic or anaerobic?

The Bacillus species used in Chrisal Probiotic products are known to be very capable in processing all kinds of organic dirt. They are high performing because they can both function under aerobic and anaerobic conditions, called facultative anaerobic.

26How do I store PIP products?

Since there are living bacteria present in PIP products, they must be stored between 5ºC and 40ºC, avoiding direct sunlight.

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